Cardiac Electrophysiology Experimental Lab

Biomodels

heart failure (acute, chronical), myocardioal ischemia (acute), renal failure (chronical), ARDS (acute, subacute). Precapillary pulmonary hypertension.

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Head

Researches

PhD. stud.

Staff:

Otomar Kittnar, MD., Prof., CSc.

 Vladimír Hrachovina, Eduard Kuriščák, Mikuláš Mlček, prof. Petr Neužil, Tomas Svoboda

Pavel Hála, Matěj Hrachovina, Jaroslav Kudlička, Stano Lacko, Michal Otahal, Michaela Popková

Alena Ehrlichová, Jana Bortelová

• Hemodynamics and myocardial performance during mechanical cardiac support operation. Systems and organ perfusion during extracorporeal life support; interaction (adverse effects) of failing heart and mechanical cardiac support; Mechanical support of critical heart failure. Dynamic and early markers of circulatory decompensation.
• Mild therapeutic hypothermia. Changes in cardiac electrophysiology (arrhythmia susceptibility, defibrillation threshold) and plasma electrolytes during early phases of cooling and hypothermia.
• Stress hormones activity during cardiac catheterization procedures
• Cardiac electrophysiology studies: ventricular fibrillation inducibility in hypothermia and renal denervation, defibrillation success prediction,
• ARDS. Interaction of hemodynamics and respiratory parameters during severe ARDS and aggressive ventilation support.

• Electrophysiology: endocardial electroanatomical mapping (Carto, NavX), Body surface potential mapping (Biosemi).
• Hemodynamics: Intravascular flow velocity (Volcano), perivascular flowmetry (Transonic), High fidelity pressure sensors (Scisense/Transonic), Cardiac P-V loop (Scisense/Transonic), Microcirculation dark field spectroscopy (Microscan), Continuous cardiac output (Edwards Lifesciences)
• Oximetry. NIRStissue oximetry (INVOS, Covidien), Mixed venous oximetry (Vigilance, Edwards Lifesciences]
• Neuroelectrophysiology EEG, SSEP (Endeavor, Nicolette)
• Imaging: Fluoroscopy (GE 8800), Echocardiography (TTE - Transthoracic Echocardiography, ICE – intracardiac echocardiography, Siemens Acuson), IVUS – intravascular ultrasound (Volcano)
• Other: Realtime blood gas analysis (Terumo CDI)

1. Coronary versus carotid blood flow and coronary perfusion pressure in a pig model of prolonged cardiac arrest treated by different modes of venoarterial ECMO and intraaortic balloon counterpulsation. By Belohlavek, Jan; Mlcek, Mikulas; Huptych, Michal; et al.: Critical Care 2012 16 (2)

2. Direct Comparison of Percutaneous Circulatory Support Systems in Specific Hemodynamic Conditions in a Porcine Model. By: Ostadal, Petr; Mlcek, Mikulas; Holy, Frantisek; et al.: Circulation-Arrhythmia and Electrophysiol. 2012, 5 (6), 1202-1206

3. Inducibility of ventricular fibrillation during mild therapeutic hypothermia: electrophysiological study in a swine model. By: Kudlicka J, Mlcek M, Belohlavek J, Hala P, Lacko S, Janak D, Havranek S, Malik J, Janota T, Ostadal P, Neuzil P, Kittnar O.: J Transl Med. 2015;13:429.

4. Renal denervation decreases effective refractory period but not inducibility of ventricular fibrillation in a healthy porcine biomodel: a case control study By: Lubanda, Jean-Claude; Kudlicka, Jaroslav; Mlcek, Mikulas; et al.: J Translat Med 2015; 13, 4

5. Median frequencies of prolonged ventricular fibrillation treated by V-A ECMO correspond to a return of spontaneous circulation rate By: Havranek S, Belohlavek J, Mlcek M, Huptych M, Boucek T, Svoboda T, Fichtl J, Hrachovina M, Linhart A, Kittnar O: Int J Artif Organs 2014; 37 (1), 48-57

6. Hemodynamic and Metabolic Parameters During Prolonged Cardiac Arrest and Reperfusion by Extracorporeal Circulation. By: Mlcek, M.; Ostadal, P.; Belohlavek, J.; et al. Physiol Res. 2012; 61 (S2), S57-S65

7. Are there any differences between stress hormone levels in non-stress conditions and in potentional stress overload (heart catheterisation) in sows? Skarlandtová H, Bičíková M, Neužil P, Mlček M, Hrachovina V, Svoboda T, Medová E, Kudlička J, Dohnalová A, Havránek Š, Kazihnítková H, Máčová L, Vařejková E, Kittnar O. : Physiol Res. 2014;63(6), 733-41.