Laboratory of Neurophysiology and Behaviour Neuroscience
Head: Jaromír Mysliveček, MD., Prof., Ph.D.
Researches: Vlado Farár, PharmDr.,Ph.D.
Staff: Kateřina Janišová, Ing.
Research is based on the:
- G protein coupled receptors, their role, regulations (muscarinic and adrenergic receptors, dopamine receptors) and interactions with other receptors (glucocorticoid receptors)
- Cholinergic synapses (acetylcholinesterase and butyrylcholinesterase activity), cholinergic signaling pathway (adenylyl cyclase, proteinkinase A, phospholipase C, NO synthase, proteinkinase C),
- Stress response, the role of M2 muscarinic receptors (telemetric monitoring of heart rate, activity and temperature)
Animal models
M2 KO ( M2 muscarinic receptor KO), CRH KO (CRH receptor knockout), M4 KO (M4 receptor knockout), PRiMA KO (acetylcholinesterase anchor protein knockout)
The restraint stress effects on behavior (Any Maze)
Selected Publications:
Farar, V., A. Hrabovska, E. Krejci and J. Myslivecek. "Developmental Adaptation of Central Nervous System to Extremely High Acetylcholine Levels." PLoS One 8, no. 7 (2013): e68265.
Farar, V., F. Mohr, M. Legrand, B. Lamotte d'Incamps, J. Cendelin, J. Leroy, M. Abitbol, V. Bernard, F. Baud, V. Fournet, P. Houze, J. Klein, B. Plaud, J. Tuma, M. Zimmermann, P. Ascher, A. Hrabovska, J. Myslivecek and E. Krejci. "Near-Complete Adaptation of the Prima Knockout to the Lack of Central Acetylcholinesterase." J Neurochem 122, no. 5 (2012): 1065-80
Tomankova, H., P. Valuskova, E. Varejkova, J. Rotkova, J. Benes and J. Myslivecek. "The M2 Muscarinic Receptors Are Essential for Signaling in the Heart Left Ventricle During Restraint Stress in Mice." Stress, (2015): 1-43.
Benes J., Varejkova E., Farar V., Novakova M., Myslivecek J.: Decrease in heart adrenoceptor gene expression and receptor number as compensatory tool for preserved heart function and biological rhythm in M2 KO animals. N-S Arch Pharmacol 385: 12, pp 1161-1173, 2012
Grants: GAUK 4806/2015; GAUK 3283/2015